The Lakes Entrance Oil Shaft is situated in allotment 21, Parish of Colquhoun, about 2 miles north-east of Lakes Entrance township. The shaft was sunk with the object of developing, by means of low pressure mining methods, the oil-bearing beds known to exist in the lower portion of the Tertiary basin in the Lakes Entrance area. It is estimated that, during mining operations, approximately 12,000 tons of Tertiary sediments were excavated. Consequently a unique opportunity was afforded the palaeontologist and geologist to study the stratigraphic sequence of Tertiary beds in the Lakes Entrance area. During visits paid to the scene of operations, extensive collections of fossiliferous material were made by the author. This report comprises notes on the scope of the micro-palaeontological investigation, the stratigraphy and the palaeontology of the Lakes Entrance Oil Shaft.