This dataset contains the result of a biological baseline survey of the port region of Eden, NSW, carried out in November 1996 by CSIRO Marine Research Centre for Research on Introduced Marine Pests (CRIMP). Collection methods employed include pylon scrapings, sediment cores, crab traps, plankton nets, and qualitative visual inspection and photographs (both still and video). Voucher specimens have been incorporated into collections of CMR, Hobart. Taxonomic groups surveyed include marine invertebrates, fishes, phytoplankton, macroalgae, and marine vegetation. This dataset forms part of a series of Port Surveys conducted by CRIMP over the period 1996 to present.